This view will show current sample’s fluorescent parameters in a grid of either histograms (ALLBY) or NxN bivariate plots.You can use the heatmap to quickly identify the highest/lowest spills of your matrix, which can be useful to track down compensation problems. The color coding is a simple heat map – blues are negative spills, and the increasingly darker shades of yellow are applied to higher values.Modifier keys can be used to accelerate the change – hold down, or, or for finer/coarser changes. You can click inside a field and use up/down arrows to change the numbers. The numbers correspond to the spill values between two parameters.You can uncheck some parameters and then use the “show all” checkbox on top to hide unchecked parameters. The checkboxes on the left control which parameters are being used in the display portion (section 3 below).The provides the capability to apply the current selected matrix to a sample or group by dragging and dropping.The Edit button will duplicate the selected matrix and allow you to edit the compensation spillover values.This can make or break an experimental analysis that spans multiple runs, we recommend using a strict naming convention for your matrices to aid in identifying which data set they belong to. The name field tells you which matrix is currently being edited.Since this window is complex, let’s break it up into 3 pieces and discuss them each below: The matrix editor window can be accessed in two ways:ġ) by double-clicking the compensation badge from the workspace window (any compensated sample has this badge next to the sample in the left hand column down the workspace.)Ģ) by clicking the “View Matrix…” button in the main Compensation window. Starting with version 10 of FlowJo, there is a new interface for viewing compensation matrices – the Matrix Editor.
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